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在韩国仁川分离Tuber himalayense的黑色素抑制作用在韩国仁川分离

Byeong Min Choi¹, Minkyeong Kim², Hyehyun Hong¹

¹Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University, Chungnam 31460, Republic of Korea.

Journal of microbiology and biotechnology

|March 13, 2024

概括

山提取物有效地抑制皮肤细胞中黑色素的产生,没有毒性. 这种天然化合物向关键的酶和参与黑色素发生的途径,显示了皮肤美白化品的潜力.

科学领域:

皮肤病学皮肤病学
化品科学化品科学
自然产品化学自然产品化学

背景情况:

对皮肤美容和抗黑色素产品越来越感兴趣.
黑色素生成,黑色素合成的过程,对于皮肤颜色是至关重要的.
铁酶 (TYR),MITF,TRP-1和TRP-2等关键酶调节了黑色素的产生.

研究的目的:

为了研究Tuber himalayense提取物的抗黑色素效应.
评估T. himalayense提取物对B16F10黑色素瘤细胞的安全性和有效性.
阐明在提取物对黑色素合成的影响背后的分子机制.

主要方法:

用T. himalayense提取物治疗α-黑色素细胞刺激激素 (α-MSH) 治疗的B16F10黑色素瘤细胞.
评估细胞活力以确定毒性.
确定黑色素,TYR,MITF,TRP-1和TRP-2水平的量化.
对基因激活蛋白激酶 (MAPK) 途径激活的分析 (ERK,JNK,p38).

主要成果:

T. himalayense提取物对B16F10黑色素瘤细胞没有表现出任何毒性.
在25,50和100微克/毫升度下观察到显著抑制黑色素合成.
提取物剂量依赖地抑制了黑色素,TRP-1,TRP-2,铁酶和MITF.
泰马拉尼斯基提取物抑制了MAPK信号通路,包括ERK,JNK和p38.

结论:

山提取物具有显著的抗黑色素原特性.
提取物通过参与黑色素发生的多个分子点起作用.
提取T. himalayense提取物是开发新型皮肤美白化品成分的有希望的候选人.

关键词:

B16F10 黑色素瘤细胞这就是MAPKK.美国MITF MITF 黑色素的产生是黑色素的产生.结核喜马拉雅任何 α-MSHH 的情况.

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