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大麦AGO4蛋白在异构补充中显示重叠的功能,具有明显的小RNA结合特性

Fabio Miloro^1,2, András Kis¹, Zoltán Havelda^1,2

¹Hungarian University of Agriculture and Life Sciences (MATE), Institute of Genetics and Biotechnology, Gödöllő, Hungary.

Plant cell reports

|March 14, 2024

概括

大麦阿尔戈诺特4 (AGO4) 蛋白在阿拉比多普西斯中表现出保存和分离的功能,补充表观遗传基因调节和结合小RNAs. HvAGO4A 和 HvAGO4B 呈现出不同的核酸偏好,影响了可转移元素的调节.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
植物科学植物科学

背景情况:

在RNA指导的DNA甲基化 (RdDM) 中,阿尔戈诺特4 (AGO4) 的作用已在Arabidopsis thaliana中得到充分研究.
它在单种植物中的功能,特别是管理大量可转移元素 (TE) 的大型基因组,仍然不太了解.
研究大麦AGO4蛋白质为人们提供了对保存的RdDM机制的见解,以及在表观遗传学和作物改进方面的潜在应用.

研究的目的:

为了识别和描述大麦AGO4基因 (HvAGO4a和 HvAGO4b).
为了研究它们在RdDM和TE调节中的功能,使用Arabidopsis的异质补充系统.
为了确定大麦AGO4蛋白质的小RNA (sRNA) 结合特异性.

主要方法:

对RNA测序数据的生物信息分析以识别HVAGO4a和HVAGO4b.
使用大麦AGO4蛋白质,对Arabidopsis ago4-3突变的异构补充.
分析sRNA结合偏好和对TE调节的影响 (例如,ONSEN逆转移素).

主要成果:

确定了两个活跃的大麦AGO4基因,HVAGO4a和HVAGO4b.
这两种蛋白质都补充了Arabidopsis ago4-3突变体,恢复了目标基因的表观遗传调节.
HvAGO4蛋白主要与24-nt sRNA结合,并触发甲基化.
与HVAGO4A对腺素 (A) 的严格偏好相比,HVAGO4B显示了更广泛的5'核酸结合 (A,G,U,C).
两种大麦AGO4蛋白都恢复了异染色体DNA的野生类型水平和ONSEN逆转移子的转录丰度.

结论:

在RdDM和TE调节中,大麦AGO4蛋白与阿拉比多普西斯AGO4具有功能性保护.
在 HvAGO4A 和 HvAGO4B 之间存在明显的 sRNA 5' 核酸结合特异性.
这些发现突显了AGO4在单种植物和双种植物中的保留和分离作用,这对表观遗传学和作物育种有意义.

关键词:

阿拉比多普西斯塔利亚纳.这种植物叫Hordeum vulgare.阿尔戈纳特4 (AGO4) 的热应激是一种热应激.通过RNA指导的DNA甲基化.小RNAs 是一个小RNA.可转移的要素是可以转移的.

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