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相关概念视频

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
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器官分段:基于深度学习的器官分段,以量化CFTR依赖的液体分泌量.

Juliet W Lefferts1,2, Suzanne Kroes1,2, Matthew B Smith1,2,3

  • 1Department of Pediatric Respiratory Medicine, Wilhelmina Children's Hospital, University Medical Center, Utrecht University, 3584 EA, Utrecht, The Netherlands.

Communications biology
|March 14, 2024
PubMed
概括
此摘要是机器生成的。

一个新的深度学习工具,OrgaSegment,准确地细分和量化器官胀. 这种方法有助于研究囊性纤维化 (CF) 和评估CF跨膜导电调节器 (CFTR) 调节药物.

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相关实验视频

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科学领域:

  • 生物医学工程 生物医学工程
  • 细胞生物学 细胞生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 患者衍生器官 (PDO) 对于临床前研究,药物开发和精密医学至关重要.
  • 在PDO中表皮液运输的评估是通过测量灯光尺寸的变化来评估,这对于研究诸如囊性纤维化 (CF) 等疾病至关重要.
  • CF是由CF跨膜导电性调节器 (CFTR) 基因的突变引起的,影响离子通道功能.

研究的目的:

  • 引入OrgaSegment,这是一个深度学习模型,用于从明亮场图像中对肠道PDO进行细分.
  • 为了准确量化有机体胀的数量,以应对各种刺激.
  • 为了促进研究CFTR功能和PDO的药物反应.

主要方法:

  • 开发了基于MASK-RCNN的深度学习模型OrgaSegment.
  • 应用 OrgaSegment 在明亮场显微镜图像中对个人肠道 PDO 结构进行细分.
  • 使用的OrgaSegment用于量化药物诱导的胀试验中的有机体胀.

主要成果:

  • OrgaSegment成功地对球形和不规则形状 (CF的标志) 的器官进行了细分.
  • 该工具可以直接量化有机体胀.
  • OrgaSegment有效地区分了具有不同CFTR突变的有机体,并测量了对CFTR调节药物的反应.

结论:

  • OrgaSegment是一种有效的,无标签的工具,用于分析有机体胀.
  • 这种方法可以推进对有机体中CFTR介导的液体分泌和其他上皮质运输机制的研究.
  • OrgaSegment支持药物开发和精密医学应用,用于CF和相关疾病.