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基质诱导的凝结激活了植物TIR域蛋白
Wen Song1,2,3, Li Liu2,4, Dongli Yu2,5
1State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, China.
Nature
|March 14, 2024
概括
植物免疫受体称为Toll/interleukin-1受体 (TIR) 域蛋白通过自我组装激活免疫力. 基质结合,如NAD+和ATP,触发了这种相分离,对植物防御至关重要.
科学领域:
- 植物免疫力
- 分子生物学
- 生物化学
背景情况:
- 植物核酸结合重复蛋白 (NLR) 免疫受体具有Toll/interleukin-1受体 (TIR) 域.
- TIR域对于识别病原体和启动植物免疫是至关重要的.
- 对于TIR域蛋白的激活机制,特别是截断的形式,人们对其了解甚少.
研究的目的:
- 阐明植物TIR域蛋白的激活机制.
- 研究基质结合在TIR域蛋白激活中的作用.
- 了解TIR域蛋白如何赋予植物免疫力.
主要方法:
- 使用纯化的TIR域蛋白进行体外相位分离试验.
- 生物化学测试以检测基质结合 (NAD+,ATP).
- 在植物实验中,在病原体接种时监测TIR域蛋白质的凝结.
- 局部定向的突变发生以破坏自我关联接口和无序区域.
主要成果:
- 在体外,NAD+与ATP的结合会诱导TIR域蛋白的相分离.
- 作为对病原体攻击的反应,TIR域蛋白在植物中形成凝结物.
- 阶段分离由保存的自我关联接口和内在无序的循环介导.
- 破坏TIR凝结物会影响TIR域蛋白的细胞死亡活动.
结论:
- 阶段分离是激活植物TIR域蛋白的关键机制.
- 基板结合通过凝结物形成触发TIR信号的自主激活.
- 这种机制提供了植物如何获得对病原体的免疫力.
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