一种益生菌向胆酸代谢,通过减少结合胆酸来缓解性结肠炎
Fan Jingjing1, Jin Weilin2, Su Shaochen3
1Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China.
来自发酵食品的益生菌可以改变胆酸代谢以改善性结肠炎 (UC) 症状. 这项研究表明,一种特定的菌株通过修改肠道细菌和胆汁酸来缓解小鼠的UC.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 代谢学 代谢学 代谢学
背景情况:
- 肠道微生物群 (GM) 失调和改变胆酸 (BA) 代谢与性结肠炎 (UC) 有关.
- 具有针对UC的特定BA-代谢能力的益生菌的治疗潜力仍未得到研究.
研究的目的:
- 探索一种来自发酵食品的新型益生菌菌株GR-4在改善UC症状方面的有效性.
- 研究GR-4对肠道微生物群组成和胆酸代谢在UC小鼠模型中的影响.
主要方法:
- 从"江苏"中分离了GR-4菌株,并评估了其BA-deconjugating活性.
- 诱导硫酸德克斯 (DSS) 的UC小鼠被用GR-4菌株治疗.
- 评估了UC症状严重程度,结肠形态,炎症,氧化应激和肠道屏障功能.
- 用代谢学和16S rRNA测序分析了肠道微生物群组成和便BA概况.
主要成果:
- 株GR-4表现出常见BA (>69%和>98.47%) 的显著脱.
- 在小鼠中,GR-4的使用缓解了UC症状,改善了结肠形态,减少了炎症和氧化应激.
- 治疗效果取决于完整的开始性微生物群.
- GR-4调节了BA配置文件,减少了合BA和增加了二次BA,并选择性地丰富了BA代谢细菌.
结论:
- 来自天然发酵食品的益生菌可以有效地操纵BA成分以减轻UC.
- 这种方法为管理肠道健康和治疗UC提供了一个有希望的策略,通过向肠道微生物群和BA代谢来治疗UC.
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