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在番茄果实发育过程中,SlmYC2促进了SlLBD40介导的细胞扩张

Lun Liu^1,2, Jialong Zhang¹, Jiayi Xu¹

¹College of Horticulture, China Agricultural University, Beijing, 100193, China.

The Plant journal : for cell and molecular biology

|March 14, 2024

概括

番茄果实的生长是由SlLBD40和SlmYC2.2.之间的合作调节的. 这种相互作用促进了细胞扩张,增强了水果的整体尺寸,并为水果发育提供了新的见解.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学

背景情况:

侧面器官边界域 (LBD) 蛋白质是植物特有的转录因子,参与生长和应激反应,对II亚家族的研究有限.
米酸信号传递的关键调节者SlmYC2在番茄果实中表达很高,影响成熟和抗病能力,但其在果实扩张中的作用尚不清楚.

研究的目的:

为了研究SLBD40的作用,一个亚家族II LBD基因,在番茄果实扩张.
阐明SlLBD40和SlmYC2在调节水果大小方面的相互作用.

主要方法:

番茄植物中的基因过度表达和淘汰.
对中骨细胞扩张和果实生长的分析.
酵母两杂交试验和共同免疫沉以研究蛋白质相互作用.
促进体结合测定和基因表达分析 (qRT-PCR).

主要成果:

过度表达SLBD40通过增强中骨细胞扩张促进了果实生长;SLBD40淘汰抑制了这一过程.
绝杀SlmYC2显著降低了果细胞扩张,表明其至关重要的作用.
在细胞扩张中SlLBD40的功能取决于SlMYC2的表达.
SlLBD40和SlmYC2的共同表达协同激活了扩展基因SlexPA5,增加了水果的大小.
SlLBD40与SlmYC2相互作用,增强了它的稳定性和丰度.
SlMYC2直接激活了SLBD40表达,这对于SLBD40介导的果实扩张至关重要.

结论:

SlLBD40和SlmYC2形成了一个促进番茄果实扩张的监管复合体.
这种相互作用增强了SLEXPA5的表达,导致细胞扩张和果实大小的增加.
这项研究揭示了一种通过SlMYC2-SlLBD40通路控制水果生长的新型分子机制.

关键词:

斯莱克斯帕5 5 在线观看在SLBD40SLBD40SLBD40中使用这就是SLMYC2的意思.细胞扩张细胞扩张果实大小果实大小果实大小蛋白质相互作用蛋白质相互作用在这里,我们可以看到子子子.

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