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Updated: Jun 24, 2026

Visualizing Bacteria in Nematodes using Fluorescent Microscopy
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现场杂交RNA光 (FISH) 作为一种可视化的方法,用于在C.菌群中的细菌殖民. 优雅的人 肠肠肠肠肠

Kayla M Poirier1, Robert J Luallen1, Dalaena E Rivera1

  • 1Department of Biology, San Diego State University, San Diego, California, United States.

microPublication biology
|March 14, 2024
PubMed
概括

在Caenorhabditis elegans中可视化活细菌对于宿主微生物研究至关重要. 与光蛋白质方法不同的是,RNA光 in situ杂交 (RNA FISH) 可靠地区分C. elegans肠道中的活体和死体细菌.

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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...