使用环境抹布用于干表面生物膜回收的效率和新性
Fergus Watson1,2, Sandra Wilks1,3, John Chewins2
1School of Biological Sciences, University of Southampton, Southampton, UK.
Access microbiology
|March 14, 2024
概括
环境抹布可以检测医疗机构的干表面生物膜 (DSB),但泡抹布最有效,可恢复30%的污染物. 目前的方法可能低估了微生物的存在,影响了临床应用.
科学领域:
- 微生物学 微生物学
- 医疗流行病学 医疗流行病学
- 感染控制 感染控制
背景情况:
- 干表面生物膜 (DSB) 在医疗保健机构中广泛应用于经常触摸的物品上.
- 对于DSB的常见检测方法的恢复效率尚不清楚.
- 生物膜很难去除,可能是医疗保健相关感染的来源.
研究的目的:
- 评估不同的环境片材料的回收效率,以检测DSB.
- 要量化微生物负载被移除,并在擦拭后留在表面.
- 评估抽样效率对低估微生物污染的影响.
主要方法:
- 使用滴流反应器和干燥循环生成了Acinetobacter baumannii的DSB.
- 通过显微镜和可活板数量确认了生物膜的存在和量化.
- 用缓冲剂预湿的泡,粘合剂和棉被用于取样DSB污染的表面.
主要成果:
- 泡顶的抹布显示出最高的回收效率,平均检测到30%的原始DSB污染.
- 粘合剂和棉的回收率明显较低,分别为6%和3%.
- 不过,在不同类型的抹布中,在表面上剩余的微生物度上没有发现显著差异,这表明释放能力可变.
结论:
- 环境扫描可以检测DSB,但它们的效率有限可能导致低估微生物污染.
- 泡抹布在DSB检测方面优于粘合剂和棉花.
- 考虑抽样回收效率对于准确的临床评估和感染控制至关重要.
相关概念视频
01:18Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
01:30Biological Treatment of Effluent and Waste Water
Biological wastewater treatment relies on the metabolic activity of microorganisms to remove pollutants from sewage. In modern treatment systems, this process is organized into sequential stages that progressively reduce solid material, dissolved organic matter, and microbial contamination. Each stage plays a distinct role in improving water quality and preparing the effluent for safe discharge or reuse.Primary and Secondary TreatmentPrimary treatment is a physical process that removes large...