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在膀癌中对信使RNA和长非编码RNA进行全转录组范围的1-甲基氨酸功能分析

Jian-Jian Yin¹, Yan-Liang Song², Yu-Feng Guo³

¹Department of Pharmacology, School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China.

Frontiers in genetics

|March 14, 2024

概括

这项研究揭示了N1-甲基氨酸 (m1A) RNA修饰是膀癌 (BLCA) 的关键. 向TRMT61A影响m1A模式,影响癌症进展和患者的存活率.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

转录后RNA修饰在癌症发展中至关重要.
N1-甲基氨酸 (m1A) 是一种重要的RNA修饰,但其在膀癌 (BLCA) 中的作用尚不清楚.
在BLCA中研究m1A在信使RNA (mRNA) 和长非编码RNA (lncRNA) 中至关重要.

研究的目的:

研究m1A修饰在mRNAs和lncRNAs在膀癌中的作用.
为了确定TRMT61A淘汰对BLCA中的m1A配置文件的影响.
探索m1A调节的转录在BLCA瘤发生中的功能影响.

主要方法:

使用甲基化RNA免疫沉降测序 (meRIP-seq) 和RNA测序 (RNA-seq).
TRMT61A在BLCA细胞中被稳定地击倒,以评估其对m1A的影响.
进行了生物信息分析,包括基因本体学和KEGG通路分析.

主要成果:

在TRMT61A淘汰后,观察到mRNA和lncRNAm6A甲基化的显著变化.
m1A调节的转录物参与蛋白质结合和癌症信号通路.
特定的m1A修饰转录 (SCN2B,ENST00000536140) 在BLCA中表达高,并与较差的患者存活率有关.

结论:

在BLCA中,TRMT61A的淘汰会显著改变m1A的景观.
m1A的mRNAs和lncRNAs的修饰在BLCA进展中起着重要的作用.
这项研究提供了关于膀癌表皮转录体调节的见解.

关键词:

膀癌:膀癌是一种癌症.在ncRNA中,我们可以 m1A1A 在线观看它们是mRNARNA.瘤发生的起源

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