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卡廷加,亚马逊和大西洋森林作为微生物细胞分解酶的天然来源

Vitor Baptista Ferrari¹, Lidiane Maria Dos Santos Lima¹, Kelly de Matos Marques¹

¹Department of Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil.

Archives of microbiology

|March 14, 2024

概括

来自巴西生物体的行为菌素显示出强大的纤维细胞分解酶,其中AC171菌株表现出高的乳酶和过氧化酶活性. 这些酶在各种条件下稳定,为生物质转化中的工业应用提供了潜力.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
工业酶工业酶工业酶

背景情况:

巴西的生物圈拥有多样化的微生物,包括以生产生物活性化合物和工业酶而闻名的actinomycetes.
像乳糖酶,酸过氧化酶和酸过氧化酶这样的纤维细胞分解酶对于降解植物生物质至关重要.
这些酶在分解红素和纤维素方面发挥着至关重要的作用,这些过程对于生物炼油厂和生物燃料生产至关重要.

研究的目的:

评估从巴西卡廷加,大西洋森林和亚马逊森林生物群中分离出来的行为菌体的纤维细胞分解潜力.
用于选产生乳糖酶 (Lac),酸过氧化酶 (LiP),酸过氧化酶 (MnP) 和细胞酶的actinomycete菌株.
为潜在的工业应用评估关键酶的稳定性和特性.

主要方法:

从土壤和根球样本中分离和选了173种actinomycete菌株.
酶测定乳糖酶,红素过氧化酶,过氧化酶和细胞酶活性.
在不同温度,pH值和盐度度下评估酶稳定性.

主要成果:

十一个actinomycete菌株显示出显著的酶性能.
菌株AC171表现出高活性:Lac为29.7U/L,LiP为2.5U/L,MnP为23U/L,在pH7.4.4时细胞酶活性为71U/L.
来自AC171的酶提取物表现出适应不同pH和盐条件的适应性,这表明LiP的性特性. 菌株AC149产生性和极度性LiP.

结论:

来自巴西生物群的行为菌素是一种丰富的强效细胞分解酶的来源.
菌株AC171是工业应用的一个有前途的候选人,因为它的高酶生产和稳定性.
已识别的氨酸过氧化酶具有独特的稳定性,使其适合在生物乙醇生物炼油厂中加工纤维素生物质.

关键词:

这些是Actinomycetes.细胞质细胞质细胞质.拉卡斯 (Laccase) 是一个古老的城市.红素过氧化酶是什么?过氧化酶是一种过氧化酶.

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