将Cryptococcus neoformans暴露在低水平下会增加它的毒性
Caylin Bosch1, Barbra Toplis1, Anton DuPreez Van Staden1,2
1Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa.
概括
在低气条件下生长的Cryptococcus neoformans在Galleria mellonella幼虫的毒性增加. 这种增强的致病性与更大的细胞大小和减少巨细胞吞有关,突出显示了的作用.
科学领域:
- 菌类学 菌类学是指菌类学.
- 传染性疾病 传染性疾病
- 宿主-病原体相互作用
背景情况:
- 以前的研究表明,含量与Cryptococcus neoformans的致病性之间存在联系.
- 环境因素,如营养的可用性,可以影响微生物的毒性.
研究的目的:
- 为了研究Cryptococcus neoformans在不同水平的预暴露后体内效应.
- 为了确定的可用性如何影响真菌形态,毒性和宿主免疫反应.
主要方法:
- 在低度 (0.21 g/L NH4Cl) 和高度 (0.53 g/L NH4Cl) 的酵母碳基中培养Cryptococcus neoformans H99.
- 感染大蝶 (Galleria mellonella) 幼虫的条件的C. neoformans.
- 在幼虫血淋巴中对C. neoformans的显微镜检查和对巨吞的评估.
主要成果:
- 在低 (LN) 条件下培养的C. neoformans比在高 (HN) 条件下培养的表现出更大的毒性.
- 在体内,LN培养的细胞更大,许多细胞超过10微米,并可能经历化.
- 与HN培养细胞相比,较少的LN培养细胞被巨细胞吞,这归因于囊厚度增加.
结论:
- 的可用性显著影响Cryptococcus neoformans的毒性,细胞形态和与宿主免疫系统的相互作用.
- 在LN条件下细胞大小增加有助于增强体内毒性.
- 在体外较厚的囊可能导致巨细胞吸收减少,影响宿主防御逃避.
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