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Updated: Jul 1, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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工程的Cas12a核酶变体与增强的基因组编辑特异性的工程

Peng Chen1, Jin Zhou1, Huan Liu1

  • 1State Key Laboratory of Virology, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Department of Clinical Oncology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.

PLoS biology
|March 14, 2024
PubMed
概括

研究人员设计了一个超忠实CRISPR-Cas12a (HyperFi-As) 变体,以减少不必要的基因编辑. 这种新变种在人体细胞中显示出明显较低的脱效应,提高了CRISPR技术的精度.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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