内源逆转录病毒HERVH衍生的lncRNAUCA1可以控制人类热囊细胞的发育
Xuhui Kong1,2, Ruiqi Li3,4,5, Manqi Chen1,2
1Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
概括
人体内源逆转录病毒 (HERVs) 在胎盘发育中发挥着新的作用. 由HERV衍生的lncRNA UCA1促进了热囊细胞干细胞的增殖,是早期发病的孕前的潜在生物标志物.
科学领域:
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 内源逆转录病毒 (ERV) 在哺乳动物的胎盘中被重新激活,并被建议调节细胞基因表达.
- 除了增强剂活动之外,ERVs在人类热囊细胞发育中的作用尚不清楚.
- 人体内源性逆转录病毒-H (HERVH) 已知可以维持表皮质多能性.
研究的目的:
- 研究HERVH在人类热细胞系发展中的作用.
- 探索 HERVH 衍生非编码RNA 在 trofhoblast 生物学中的功能.
- 评估 HERVH 元素作为胎盘疾病生物标志物的潜力.
主要方法:
- 在人类热细胞系中对染色质可访问性的分析.
- 从HERVH衍生的长非编码RNA (lncRNAs) 的转录分析.
- 使用带有异位UCA1表达的人类热原体干细胞 (hTSC) 的功能研究.
- 来自UCA1-过度表达细胞的转录组和先兆子的胎盘样本的比较.
主要成果:
- LTR7C/HERVH亚家族在人类热囊细胞中显示出可访问的染色质.
- 由HERVH衍生的lncRNA UCA1促进了hTSC的增殖,并抑制了合成化.
- 上调的UCA1被发现在早期发病的孕前期胎盘中,与疾病样本的转录组相似.
结论:
- HERVH在人类热囊细胞发育中发挥着独特的作用,超出了增强剂活性.
- 来自HERVH的灵长类特异性lncRNA UCA1调节了 trofhoblast 的增殖和分化.
- 升级的UCA1可以作为早期发作的孕前的生物标志物,将ERV与胎盘综合征联系起来.
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