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相关概念视频

Ligand Binding Sites02:40

Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

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Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K
Activation of Integrins01:15

Activation of Integrins

3.4K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
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Ligand Binding and Linkage00:49

Ligand Binding and Linkage

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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相关实验视频

Updated: Jul 1, 2025

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

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结构洞察力揭示了LAG-3同位体化,带结合和功能之间的相互作用.

John L Silberstein1,2, Jasper Du3, Kun-Wei Chan4

  • 1Program in Immunology, Stanford University School of Medicine, Stanford, CA 94305.

Proceedings of the National Academy of Sciences of the United States of America
|March 14, 2024
PubMed
概括

淋巴细胞激活基因-3 (LAG-3) 通过域2进行二分化对于其抑制功能至关重要. 准LAG-3域可以阻止连接体结合,并提供新的免疫疗法策略.

关键词:
现场行动组-3癌症免疫疗法免疫疗法分化二元化是指二元化的过程.免疫检查点是一个检查点.结构生物学结构生物学

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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

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Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
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Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

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相关实验视频

Last Updated: Jul 1, 2025

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
08:40

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

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Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
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Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

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科学领域:

  • 免疫学 免疫学 免疫学
  • 结构生物学 结构生物学
  • 癌症免疫疗法癌症免疫疗法

背景情况:

  • 淋巴细胞激活基因-3 (LAG-3) 是T细胞上的抑制受体,也是免疫治疗的关键标.
  • 据认为,LAG-3的Domain 1 (D1) 是与MHCII和FGL1配体的主要相互作用部位.

研究的目的:

  • 阐明LAG-3功能的结构基础,并确定新的治疗点.
  • 调查LAG-3二分化对其抑制活性和连接体结合的作用.

主要方法:

  • 高分辨率的结构分析的糖化小鼠LAG-3ectodomain.
  • 局部定向的突变发生来破坏二元化接口.
  • 生物化学测试以评估连体结合 (MHCII,FGL1) 和T细胞抑制.

主要成果:

  • 通过Domain 2 (D2),LAG-3经历了 cis-homodimerization,这对其功能至关重要.
  • 在二元接口中,一种新的蛋白质-甘氨酸相互作用影响了D1的方向.
  • 突变破坏D2二分化,取消了连接体结合和T细胞抑制.
  • 针对D1,D2和D3域的抗体阻断了二分化和配体结合,表明了全调节.

结论:

  • 由D2调节的LAG-3二分化对其抑制功能和连接体相互作用至关重要.
  • 超出D1的新型表征可以作为开发基于LAG-3的免疫疗法的目标.
  • 了解LAG-3的结构和二元化为癌症治疗提供了新的途径.