视觉化小鼠卵细胞在高速冷却和防水凝阻过程中冰晶的行为
Xin Li1,2,3, Shuyong Zhang1,2,3, Yuqi Zhang1,2,3
1Institute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai, China.
Biopreservation and biobanking
|March 14, 2024
概括
了解冷却过程中的卵细胞固化是至关重要的. 这项研究开发了一种可视化冰形成的方法,使卵子细胞在较低的冷保护剂度下进行玻璃化.
科学领域:
- 生殖生物学 生殖生物学
- 低温生物学 低温生物学
- 生物物理学的生物物理.
背景情况:
- 卵细胞玻璃化是一种常见的临床技术.
- 在超快速冷却卵细胞的过程中固化的过程还不太清楚.
- 结晶会影响冷保存期间的卵细胞存活.
研究的目的:
- 在冷却和加热过程中建立一个观察卵细胞结晶的系统.
- 为了确定成功卵细胞玻璃化所需的最小冷保护剂 (CPA) 度.
- 研究新的方法来降低CPA在卵细胞冷保存中的毒性.
主要方法:
- 开发了一个可视化系统,在快速冷却和变暖期间观察卵细胞中的冰结晶.
- 使用视觉方法确定CPA度的值.
- 封装在防水凝中的卵细胞用于玻璃化.
主要成果:
- 确定了CPA度,可以在高速冷却 (2821.58°C/分钟) 过程中防止冰的形成.
- 在图像序列中没有观察到冰面,图像序列中有16.5%的DMSO + 16.5%的EG.
- 实现了水凝封装卵细胞的玻璃化,CPA降低 (2.4M总透CPA),没有形成冰晶.
结论:
- 这项研究提供了一种可视化和理解玻璃化过程中卵细胞凝固的方法.
- 通过使用防水凝进行卵细胞玻璃化,降低CPA度是可行的.
- 这项研究为更安全,更有效的卵子冷保存提供了潜力.
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