骨髓衍生生长因子是基-1-酸盐受体2的配体吗?
Yong-Shan Zheng1, Ya-Li Liu2, Zeng-Guang Xu2
1Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China; Shanghai Institute of Biological Products Co., Ltd., Shanghai, China.
Biochemical and biophysical research communications
|March 14, 2024
概括
分泌性骨髓衍生生长因子 (MYDGF) 不会激活氨酸-1-酸盐受体2 (S1PR2). 这项研究没有发现MYDGF与S1PR2结合或激活的证据,这挑战了之前的发现.
科学领域:
- 细胞生物学 细胞生物学
- 分子信号传递是分子信号传递.
- 生物化学 生物化学
背景情况:
- 分泌性骨髓衍生生长因子 (MYDGF) 参与器官修复.
- 介导MYDGF作用的特定受体仍然未确定.
- 之前的研究表明,MYDGF通过黄素-1-酸盐受体2 (S1PR2) 起作用.
研究的目的:
- 在功能上验证S1PR2是否作为MYDGF的受体.
- 通过基于细胞的测定来研究MYDGF和S1PR2之间的相互作用.
主要方法:
- 利用NanoLuc二进制技术 (NanoBiT) 进行β-停滞剂招募测试.
- 雇佣的cAMP响应元 (CRE) 控制的NanoLuc记者测试.
- 在HEK293T细胞中过度表达的人类S1PR2上测试了复合的人类MYDGF和sphingosine-1-phosphate (S1P).
主要成果:
- 斯芬哥辛-1-酸盐 (S1P) 有效地激活了人类的S1PR2.2.
- 在大肠杆菌或HEK293细胞中产生的复合人类MYDGF,对S1PR2.2.没有可检测的影响.
- 这些发现表明MYDGF不是S1PR2.2的配体.
结论:
- 人类MYDGF不作为人类S1PR2.2.的连接体起作用.
- 鉴于进化保护,MYDGF不太可能是其他脊椎动物中的S1PR2联体.
- 对于MYDGF的器官修复功能的受体仍然需要确定.
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