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Updated: Jun 30, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

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微生物群蛋糕的新一块:采矿'omics用于DNA逆转状态

Danielle E Campbell1, Megan T Baldridge1

  • 1Division of Infectious Diseases, Department of Medicine, Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.

Cell host & microbe
|March 14, 2024
PubMed
概括

研究人员研究了来自炎症性肠病 (IBD) 患者的细菌体中的可逆DNA位点. 他们发现肠道细菌,它们的遗传元素和人类宿主之间存在复杂的相互作用,这凸显了对先进生物的需求.

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相关概念视频

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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