相关实验视频

Updated: Jun 30, 2025

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

自组装的基于费里的纳米颗粒引发了强大的宽频保护性免疫反应,对抗SARS-CoV-2变种

Weiqi Wang¹, Xianyong Meng², Huan Cui³

¹College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, Jilin, China.

International journal of biological macromolecules

|March 14, 2024

概括

使用欧米克朗BA.5 RBD结合的费里纳米颗粒的新型纳米颗粒疫苗策略显示,对于通用COVID-19疫苗具有前景. 这种方法在临床前研究中证明了对多种SARS-CoV-2变异的广泛保护.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
纳米技术纳米技术

背景情况:

由SARS-CoV-2引起的COVID-19大流行需要快速开发有效的,广泛的疫苗.
现有的疫苗面临着新兴变种和生产可扩展性的挑战.

研究的目的:

使用新型纳米粒子结合平台开发SARS-CoV-2的通用疫苗策略.
设计和测试Omicron BA.5受体结合域 (RBD) 结合的自组装费里纳米粒子 (NP).

主要方法:

通过化将RBD-Fc与蛋白质A-ferritin结合,以创建多价值NP.
评估NP的结构统一性,稳定性和组装效率.
评估免疫反应 (先天性,幽默性,细胞) 和黄金仓鼠模型中的保护.

主要成果:

成功创建了统一,稳定,并有效地组装RBD结合的费里NP.
证明了先天性,幽默性 (Tfh-GCB-血细胞) 和细胞性 (IFN-γ) 免疫的协同刺激.
实现了高交叉中和反应,并保护了对多个SARS-CoV-2突变菌株的仓鼠.

结论:

与RBD结合的费里丁NP平台为快速开发疫苗提供了一个有前途的通用战略.
这种方法显示出具有竞争力的免疫疗效,并有可能在下一代疫苗中广泛应用.
纳米粒子结合平台促进了高效的制造和SARS-CoV-2预防的广泛应用.

关键词:

交叉中和抗体的抗体.纳米粒子疫苗纳米粒子疫苗这就是SARS-CoV-2病毒.自组装的费里廷是自组装的

更多相关视频

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...