HPV16 E7通过AP2复合体调节MET和CD109的细胞表面表达
Oscar Trejo-Cerro1, Om Basukala2, Michael P Myers1
1International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34149, Trieste, Italy.
Tumour virus research
|March 14, 2024
概括
人类乳头瘤病毒 (HPV) 蛋白E7通过与AP2复合体相互作用,影响细胞表面蛋白水平. HPV-16 E7 失调MET和CD109的表达,影响细胞贩运途径.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 病毒学 病毒学
背景情况:
- 人类乳头瘤病毒 (HPV) 蛋白E6和E7是宫癌发展的关键因素.
- 这些瘤蛋白破坏细胞通路,包括内细胞和细胞贩运.
- HPV-16 E7 特别针对参与内细胞分裂的适应蛋白 (AP) 复合体.
研究的目的:
- 研究HPV-16E7在调节细胞表面蛋白质表达中的作用.
- 为了确定HPV-16 E7如何与AP2复合体相互作用.
- 为了阐明HPV-16 E7对MET和CD109膜蛋白表达的影响.
主要方法:
- 对NIKS细胞中细胞表面蛋白质表达的分析,这些细胞被改造为表达HPV-16 E7.
- 使用细胞测试研究HPV-16 E7,AP2,MET和CD109之间的相互作用.
- 在HPV阳性细胞系中与HPV-16 E7表达和不表达的细胞系中比较蛋白质表达.
主要成果:
- HPV-16 E7通过与AP2复合体的相互作用来调节细胞表面蛋白质.
- 在表达HPV-16 E7.7的细胞中,MET和CD109膜蛋白表达被上调.
- HPV-16 E7 破坏了MET,CD109 和 AP2 蛋白之间的相互作用.
- 缺少HPV-16 E7导致HPV阳性细胞系中MET和CD109的下调.
结论:
- HPV-16 E7显著影响细胞表面蛋白质表达和细胞贩运.
- HPV-16 E7和AP2之间的相互作用影响了MET和CD109.9的调节.
- 这些发现扩大了对HPVcoprotein功能的理解,并确定了潜在的治疗点.
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