溶解素通过HO-1通路保护糖尿病诱导的结肠氧化应激,屏障功能障碍和相关的腹
Ting Yu1, Die Chen1, Hongyan Qi1
1Department of Gastroenterology, The First Affiliated Hospital with Nanjing Medical University, Jiangsu Province, China.
BioFactors (Oxford, England)
|March 14, 2024
概括
通过保护肠道屏障,Resolvin D1 (RvD1) 可以预防糖尿病性腹. 它减少了结肠中的氧化应激和亡,恢复了紧密结合蛋白,并使便的水含量正常化.
科学领域:
- 胃肠病学 胃肠病学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 糖尿病与增加的氧化压力有关.
- 这种氧化应激会损害结肠上皮细胞,导致肠壁功能障碍和腹.
- 糖尿病腹是糖尿病患者的重要胃肠道并发症.
研究的目的:
- 研究Resolvin D1 (RvD1) 对糖尿病引起的氧化应激和肠道屏障破坏的保护作用.
- 阐明RvD1在糖尿病肠道病变的背景下作用的潜在机制.
主要方法:
- 利用小鼠模型的链毒素诱导的糖尿病和暴露于高葡萄糖的人类结肠杆菌培养.
- 评估了便参数,肠道透性,体电阻,反应性氧物种 (ROS) 生产,亡和紧结蛋白表达 (ZO-1,occludin).
- 通过使用HO-1抑制剂ZnPP,研究了Akt酸化和血氧酶-1 (HO-1) 在RvD1的保护作用中的作用.
主要成果:
- 在没有影响血糖水平的情况下,RvD1使便的水含量正常化,并预防了糖尿病小鼠的肠壁功能障碍,氧化应激和亡.
- RvD1恢复了紧结蛋白ZO-1和奥克卢丁的表达.
- RvD1治疗增加了Akt酸化和HO-1表达,其保护作用被HO-1抑制剂ZnPP废除.
- 类似的保护效应在人类的结肠状培养物中也被观察到.
结论:
- 糖尿病的氧化应激会损害粘膜屏障,导致糖尿病腹.
- 像RvD1这样的溶解素可以防止ROS介导的粘膜损伤,并保持肠道屏障功能.
- RvD1通过PI3K/Akt激活和随后的HO-1在肠上皮细胞的上调作用,为糖尿病腹提供了潜在的治疗策略.
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