Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

50.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
RNA Splicing01:32

RNA Splicing

56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Natural and evolved membrane-associated accessory proteins differentially engage SNARE machinery for AAV egress.

Journal of virology·2026
Same author

Erratum: Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors.

Molecular therapy. Advances·2026
Same author

Adeno-associated virus gene therapy-mediated CCR5 blockade suppresses virus replication long term in SHIV-infected macaques.

Science translational medicine·2026
Same author

Enhancer-directed gene delivery for digit regeneration based on conserved epidermal factors.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

In vivo site-specific engineering to reprogram T cells.

Nature·2026
Same author

A stereotactic injection method to establish a clinically relevant germinal matrix hemorrhage murine model.

Journal of neuroscience methods·2026

相关实验视频

Updated: Jun 30, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.6K

重新利用CRISPR-Cas13系统进行强大的mRNA转链剪接.

David N Fiflis1, Nicolas A Rey2, Harshitha Venugopal-Lavanya1

  • 1Department of Biomedical Engineering, Duke University, Durham, NC, USA.

Nature communications
|March 15, 2024
PubMed
概括

克里斯普尔-Cas13系统使得克里斯普尔辅助的mRNA片段转接 (CRAFT) 能够进行大型mRNA编辑. 这种新的RNA编辑工具可将RNA片段转接成内源转录.

更多相关视频

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.1K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.7K

相关实验视频

Last Updated: Jun 30, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.6K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.1K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.7K

科学领域:

  • 分子生物学分子生物学
  • 基因编辑 基因编辑
  • 在RNA生物学,RNA生物学.

背景情况:

  • 六型CRISPR酶,特别是Cas13,是RNA编辑的已知工具,包括单基编辑,脱甲基和RNA分裂.
  • 目前的RNA编辑方法在修改信使RNA (mRNA) 转录的广泛部分方面存在局限性.

研究的目的:

  • 开发和验证一种新的RNA编辑技术,即使用CRISPR-Cas13系统的CRISPR辅助mRNA片段转接 (CRAFT).
  • 通过促进外源RNA片段的转链剪接,使大量内源mRNA转录的编辑成为可能.

主要方法:

  • 重新利用CRISPR-Cas13系统进行RNA转剪接.
  • 采用分割报告测试来评估正交的Cas13系统.
  • 优化指导RNA长度,并在内基区域内确定最佳的转链剪接位.

主要成果:

  • 通过使用CRAFT方法,成功地将外源RNA片段转接成内源的mRNA前转录.
  • 在各种哺乳动物细胞类型中实现了大规模5'和3'mRNA段的显著增强编辑.
  • 在大片段的编辑效率方面,超越了现有的spliceosome介导的转拼方法.

结论:

  • CRAFT代表了一种用于实质性的mRNA转录修改的多功能平台.
  • 应用包括附加蛋白质标签,研究多个突变或SNP,修改未翻译区域 (UTR) 和替换大型mRNA段.