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在Mycobacterium Tuberculosis从临床样本的菌株中识别编码毒素-抗毒素系统的基因

Karthikeyan Sundaram¹, Leela Kagithakara Vajravelu¹, Ravichandiran Velayutham²

¹Department of Microbiology, SRM Medical College Hospital and Research Centre, Kattangulathur, Chennai, 603203, Tamilnadu, India.

Infectious disorders drug targets

|March 15, 2024

概括

这项研究在Mycobacterium结核病临床样本中确定了毒素-抗毒素基因Rv1044和Rv1045. 这些发现可能有助于理解和对抗多药耐药结核病.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

毒素-抗毒素 (TA) 系统是Mycobacterium结核病 (MTB) 中普遍存在的遗传元素,对细菌生存至关重要.
MTB 拥有多样化的 TA 基位,包括 VapBC,HigBA 和 MazEF,超过其他微生物中发现的基位.
这些系统涉及毒素和抗毒素成分之间的相互作用,以调节细胞过程.

研究的目的:

在临床Mycobacterium结核病分离物中识别和描述毒素-抗毒素系统编码的基因.
在肺结核和肺外结核样本中调查特定TA基因的存在.

主要方法:

采集和分析了临床样本 (肺结核和肺外结核),使用Ziehl-Neelsen染色和核酸放大试验.
用细菌培养,基因组DNA提取和聚合酶链反应 (PCR) 来检测特定的基因.
目标基因的放大使用PCR与特定的原始剂进行.

主要成果:

显微镜和NAAT证实了临床样本中结核菌根的存在,并量化了细菌负载.
来自培养细菌的基因组DNA在H37Rv和临床分离物中产生了Rv1044 (624bp) 和Rv1045 (412bp) 的放大产物.
观察到的片大小与这些基因的预期范围保持一致.

结论:

Rv1044和Rv1045被确定为属于TA位点的AbiEi/AbiEii家族的假设蛋白质.
这些TA编码基因在临床MTB菌株中的存在为未来研究提供了潜在的目标.
这种识别可以促进对多药耐药和广泛耐药结核病机制的调查.

关键词:

结核病菌菌菌的结核病菌的结核病菌.结核病是一种疾病.这是一种抗毒素抗毒素.病变的发生和发病.毒素毒素是一种毒素.毒素-抗毒素系统的毒素.

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