在长时间的床上休息期间,人类转录组及其昼夜组织的广泛动态变化
Simon N Archer1, Carla Möller-Levet2, María-Ángeles Bonmatí-Carrión1,3,4
1Surrey Sleep Research Centre, Faculty of Health & Medical Sciences, University of Surrey, Guildford, UK.
iScience
|March 15, 2024
概括
长期的挑战,如床上休息,会破坏人类的转录组,影响mRNA翻译和免疫功能. 这些时间变化甚至在康复后仍然存在,为适应和健康提供了洞察力.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 人体生理学 人体生理学
背景情况:
- 人类转录组表现出每日节律受到昼夜时钟,行为和系统因素的影响.
- 长期的生理挑战对人类转录组的时间组织的影响仍然在很大程度上是未知的.
- 了解这些变化可以提供有关适应,疾病和恢复过程的见解.
研究的目的:
- 为了研究90天持续的床上休息协议对人类转录组的时间组织的影响.
- 为了确定长时间的生理压力下转录组变化的程度和持续时间.
- 确定受长期挑战影响的分子途径.
主要方法:
- 分析了24小时时间序列的转录组数据,来自20名接受90天持续床上休息的个体.
- 应用缩小维度方法来识别转录组变化的模式.
- 评估节奏转录的数量,振幅和相位的变化,包括时钟基因.
主要成果:
- 一个90天的床上休息协议显著影响了91%的人类转录组.
- 转录组变化持续存在,76%的受影响的转录在恢复10天后仍然存在变化.
- 受影响的转录主要与mRNA翻译和免疫功能途径有关.
- 在转录的节律性 (数量,振幅,相位) 中观察到显著的变化,包括核心时钟基因.
结论:
- 长期的生理挑战会导致人类转录组的时间组织发生深刻而持久的变化.
- 这些发现强调了转录组对持续的压力因素的敏感性及其缓慢的恢复.
- 这些结果对了解微重力和长时间的床上休息等条件对健康的影响有意义,为潜在的对策提供了信息.
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