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从圣尼克泰尔的PDO奶酪生产区隔离的Streptococcus thermophilus的遗传和技术多样性
Anna Grizon1, Sebastien Theil1, Cecile Callon1
1UMR545 Fromage, INRAE, VetAgro Sup, Université Clermont Auvergne, Aurillac, France.
Frontiers in microbiology
|March 15, 2024
概括
来自特定奶酪地区的本土性Streptococcus thermophilus菌株表现出高度的遗传和功能多样性. 这种变异性可以被利用来开发独特的起始品种,增强奶酪的特点和地理联系.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 基因组学就是基因组学.
背景情况:
- 热球菌 (Streptococcus thermophilus) 对于快速酸化奶酪至关重要.
- 过度依赖商业品种削弱了传统奶酪的特性.
- 保护的原产地名称 (PDO) 寻求本土的开端,以保持产品的身份和感官品质.
研究的目的:
- 调查来自圣尼克泰尔原产地原名区的S. thermophilus菌株的遗传和功能多样性.
- 将本地分离物与商业菌株进行比较,以了解变异性.
- 评估这些本地菌株的潜力,作为本土的开端.
主要方法:
- 从牛奶和商业初始化剂中分离和识别S. thermophilus.
- 全基因组测序和对比泛基因组分析.
- 评估酸化和蛋白质溶解活动.
主要成果:
- 遗传学分析与地理来源没有相关性.
- 全基因组聚类揭示了五个不同的S. thermophilus分离的群体.
- 在分离物中观察到酸化活性的显著变异性和蛋白质分解活性较少的多样性.
- 辅助基因通过KEGG注释显示了参与各种功能类别.
结论:
- 高基因和功能多样性S. thermophilus存在于一个小的地理区域内.
- 这种多样性为开发特定的本土初始文化提供了潜力.
- 利用当地的S. thermophilus菌株可以提高奶酪的感官质量,并加强地理联系.
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