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患有心脏病和心脏衰竭的细胞外小囊加速瘤的生长

Tal Caller^1,2, Itai Rotem^1,2, Olga Shaihov-Teper^1,2

¹Neufeld and Tamman Cardiovascular Research Institutes, School of Medicine, Tel Aviv University, Israel (T.C., I.R., O.S.-T., D.L., Y.S., R.S., M.N., N.N.-S., J.L.).

|March 15, 2024

概括

心肌梗塞 (MI) 后心脏中的小细胞外囊 (sEV) 促进癌症生长. 使用螺旋减少了这种效应,为心脏功能障碍和癌症之间的联系提供了潜在的治疗策略.

科学领域:

心脏病学
癌症学
细胞外膀生物学

背景情况:

心肌梗塞 (MI) 和心力衰竭与癌症发生率的增加有关.
连接心脏功能障碍和癌症的根本机制尚不清楚.
心脏小细胞外囊 (sEVs) 被研究为潜在的调解器.

研究的目的:

测试心脏介质层细胞衍生的sEVs (cMSC-sEVs) 与心脏病发作后的左室功能障碍 (LVD) 与癌症的联系的假设.
分析cMSC-sEVs对癌细胞和瘤生长的影响.
评估螺旋作为一个治疗干预.

主要方法:

从MI后的心脏和培养的cMSC中净化和表征sEV.
对cMSC-EV货物的蛋白质组分析.
在体外评估cMSC-sEV对癌细胞,巨细胞和内皮细胞的影响.
在MI后LVD小鼠的体内瘤建模,包括sEV转移和耗尽研究.
对螺旋治疗的评估.

主要成果:

与没有失败的心脏相比,心脏和cMSC产生的sEV具有促进瘤的负载.
在体外和体内的肺癌和结肠癌增长加速.
在患有LVD的小鼠中,继发性转移后的cMSC- sEV增加了瘤大小和扩散.
降低了与心脏病相关的瘤增长,而螺旋乳治疗抑制了瘤的生长.

结论:

来自心脏病发作后的心脏病发作,特别是cMSC-sEV,含有前原因子.
癌细胞吸收这些SEV会增加瘤的生长.
螺旋乳治疗有效地减少了心脏病发作后瘤的加速生长,这表明了治疗途径.

关键词:

心脏瘤学细胞外囊泡纤维细胞心脏衰竭炎症发生介质干细胞心肌梗塞新生体螺旋乳

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