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Updated: Jun 30, 2025

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
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Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Published on: June 2, 2020

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在Danaus plexippus中发现功能性染色体组织的非随机模式的证据

Ashlyn Kimura1, Alwyn C Go2, Therese Markow3,4

  • 1Department of Ecology and Evolutionary Biology, University of California Irvine, Irvine, CA 92647, USA.

Genome biology and evolution
|March 15, 2024
PubMed
概括

在君主蝶中,发育阶段调节的基因形成比预期的更大,不更多的集群. 丰富的染色体显示非随机基因组织,揭示了Diptera和Hymenoptera之外的见解.

关键词:
类动物 (Lepidoptera) 是一种类动物.染色体组织是染色体组织.表达方式表达方式表达方式表达方式表达方式表达方式表达基因聚类是基因的聚类.昆虫 昆虫 是一种昆虫.性偏差表达基因的基因.

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Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes11:36

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Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
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In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
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Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
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