含有Apolipoprotein E的脂蛋白及其与LRP1的细胞外相互作用会影响LPS诱导的炎症
Shogo Akahane1, Hiroto Matsuura1, Takahiro Kaido1,2
1Department of Clinical Laboratory Investigation, 34808 Graduate School of Medicine, Shinshu University , Matsumoto 390-8621, Japan.
Biological chemistry
|March 15, 2024
概括
含有Apolipoprotein E的脂蛋白 (apoE-LP) 可以通过中和LPS,施加抗炎作用和影响LRP1通路来调节脂聚糖 (LPS) 诱导的炎症,从而提供对败血症病变的见解.
科学领域:
- 生物化学 生化学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 败血症的发病包括由脂聚糖 (LPS) 诱导的炎症和通过低密度脂蛋白受体相关蛋白1 (LRP1) 的apolipoprotein (apo) 含E脂蛋白 (apoE-LP) 代谢.
- 连接这些过程的精确机制仍然不完全理解.
研究的目的:
- 调查apoE-LP及其组分对LPS.反应中的互白素 (IL) - 6和LRP1mRNA表达的影响.
- 为了评估apoE-LP和LPS之间的结合亲和力.
主要方法:
- 人体纤维细胞用LPS和含有apoE的乳液颗粒 (apoE-EP) 进行培养.
- 量化了IL-6和LRP1的mRNA表达.
- 用光标记的LPS被用来评估与血清脂蛋白分数和apoE-EP的相互作用.
主要成果:
- LPS显著上调IL-6和LRP1的mRNA表达.
- 用apoE-EP,apoE或EP预化LPS明显抑制了这种上调.
- 作为LRP1抑制剂的乳酸铁素,apoE-EP的抗炎作用被部分降低.
- 根据IL-6抑制,ApoE-EP和富含甘油三醇的脂蛋白显示出显著的LPS亲和力,尽管低于预期.
结论:
- 由LPS诱导的炎症可能由apoE-LP的LPS中和能力调节.
- apoE的抗炎作用有助于这种调节.
- 通过LRP1介导的代谢途径在对LPS的炎症反应中发挥作用.
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