前膜皮层的金字塔神经元到腹膜体区域的投射促进了来自sevoflurane麻醉的兴奋
Fuyang Cao1,2,3, Yongxin Guo1, Shuting Guo1,3
1Department of Anesthesiology, The First Medical Center of Chinese PLA General Hospital, Beijing, China.
CNS neuroscience & therapeutics
|March 15, 2024
概括
激活前临床皮层的金字塔神经元 (PrLPyr) 和它们向腹膜区域 (VTA) 的投射,有助于从sevoflurane麻醉中出现. 这种途径通过在VTA中激活NMDA受体来影响兴奋.
科学领域:
- 神经科学是一个神经科学.
- 麻醉学 麻醉学
- 神经药理学神经药理学
背景情况:
- 尽管使用了180年,但全身麻醉机制在很大程度上仍然未知.
- 腹膜区域 (VTA) 和前皮层 (PrL) 具有显著的解剖连接性.
- 前临床皮层金字塔神经元 (PrLPyr) 投射到VTA在麻醉调节中的作用尚不清楚.
研究的目的:
- 研究PrLPyr-VTA通路在麻醉-兴奋调节中的参与.
- 确定PrLPyr-VTA投射对麻醉产生的影响背后的特定神经机制.
主要方法:
- 利用化学遗传学和光遗传学来操纵PrLPyr-VTA通路活动.
- 使用脑电图和爆发抑制比率 (BSR) 评估麻醉深度.
- 监测麻醉诱导和出现时间的右转反射;微注射NMDA/AMPA对抗剂到VTA.
主要成果:
- PrLPyr神经元和PrLPyr-VTA通路的激活延长了麻醉诱导并促进了出现.
- 对PrLPyr-VTA投影的光遗传激活延长了诱导时间,并促进了sevoflurane麻醉的出现.
- VTA NMDA受体对抗性减弱了延迟诱导,并促进了由PrL-Pyr-VTA激活引起的出现.
结论:
- 皮尔神经元和它们的VTA投射促进从sevoflurane麻醉中出现.
- PrLPyr-VTA通路通过NMDA受体激活在VTA中调解其作用.
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