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相关概念视频

Case Studies01:22

Case Studies

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There are many research methods available to psychologists in their efforts to understand, describe, and explain behavior and the cognitive and biological processes that underlie it.
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The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys.
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In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
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Free Jet01:14

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Free jets describe the flow of liquid exiting a reservoir through an opening into the atmosphere without resistance. The velocity (v) of the liquid jet is derived using Bernoulli's principle and expressed as:
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Single Pipe Systems01:24

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In pipe flow analysis, problems are typically categorized into three types — Type I, Type II, and Type III — based on the known parameters and the desired outcome. Each type of problem addresses specific engineering requirements using fluid properties, pipe characteristics, and operational conditions.
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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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维特罗喷气机:新功能和案例研究

Rene J M Henderikx1, Daniel Mann2, Aušra Domanska3

  • 1Maastricht Multimodal Molecular Imaging Institute (M4i), Division of Nanoscopy, Maastricht University, Maastricht, The Netherlands.

Acta crystallographica. Section D, Structural biology
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PubMed
概括
此摘要是机器生成的。

维特罗喷气器简化了单粒子冷电子显微镜 (cryo-EM) 样品的准备工作. 最近的改进增强了其自动化工作流程,以便在多种生物样本中进行可重复的,时间效率高的冷EM结构确定.

关键词:
这是一架VitroJet飞机.低温电磁冷却仪的使用冰的厚度 冰的厚度喷气式玻璃化器的使用方法引脚打印 引脚打印 引脚打印

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科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 单粒子冷电子显微镜 (cryo-EM) 在现代结构生物学中至关重要.
  • 传统的冷EM样品准备涉及到微妙的手动处理和转移步骤.
  • 化EM技术的技术进步需要改进样品准备方法.

研究的目的:

  • 介绍最近对VitroJet系统的技术改进.
  • 为了突出VitroJet在冷-EM工作流程中的好处.
  • 为了证明VitroJet适合于时间效率高的冷EM结构确定.

主要方法:

  • 使用等离子处理和针印刷的自动化网格准备.
  • 低体积样本沉积和光学冰厚测量.
  • 预先剪切的Autogrids通过喷气玻璃化进行冷固定.

主要成果:

  • 维特罗喷气系统经历了许多技术发展,导致广泛采用.
  • 最近的改进提高了冷EM工作流程的控制和可重现性.
  • 在各种样本类型中展示了成功的应用,包括病毒,蛋白质和纳米粒子.

结论:

  • 维特罗喷气器 (VitroJet) 便于准确且可重复的冷电磁样品制备.
  • 该系统的进步有助于节省时间的结构确定.
  • 维特罗喷气器是促进各种生物领域的冷电磁研究的宝贵工具.