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对甜菜化病毒的衰退性耐药性是由Beta vulgaris中真核转化启动因子 (iso) 4E所赋予的
Lukas Rollwage1, Hilde Van Houtte2, Roxana Hossain1
1Institute of Sugar Beet Research, Göttingen, Germany.
Plant biotechnology journal
|March 15, 2024
概括
植物真核转化启动因子 (eIFs) 是糖甜菜病毒相互作用的关键. 针对Bv-eIF(iso) 4E在Beta vulgaris减少的甜菜化病毒中,显示eIFs.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞翻译启动因子 (eIFs) 对mRNA翻译和植物病毒相互作用至关重要.
- 相互作用通常涉及植物eIFs和病毒蛋白基因组链接 (VPg) 的potyviruses.
- 失去eIF相互作用会通过阻碍病毒翻译和复制来赋予衰退性抵抗.
研究的目的:
- 为了确定Beta vulgaris (糖甜菜) 对甜菜化病毒 (BChV) 的易感性因素.
- 为了研究波列罗病毒VPgs和甜菜eIFs之间的相互作用的特异性.
- 评估eIF的潜力,作为一种资源,用于在甜菜中培育聚洛病毒耐药性.
主要方法:
- 酵母的双杂交和双分子光补充测试检测物理相互作用.
- 针对位点的突变发生,以探讨VPg-eIF相互作用的特异性.
- 通过CRISPR/Cas9基因组编辑,创建eIF淘汰糖植物.
主要成果:
- Bv-eIF(iso) 4E被确定为BChV的易感因子,与BChV-VPg相互作用.
- 甜菜轻微黄化病毒 (BMYV) 的VPg与Bv-eIF4E和Bv-eIF(iso) 4E相互作用.
- 在Bv-eIF(iso) 4E的结口袋中的突变取消了VPg相互作用,表明高特异性.
- Bv-eIF(iso) 4E淘汰植物显示显著降低了BCHV积累和感染率 (28.86%).
- 在淘汰植物中没有观察到对BMYV积累的影响.
结论:
- Bv-eIF(iso) 4E是BChV的易感性因子,突出了聚类病毒中特定的eIF-VPg相互作用.
- 针对eIF,特别是Bv-eIF{iso) 4E,是一个有前途的策略,用于在甜菜中开发波列罗病毒耐药性.
- 对eIF的进一步研究可以帮助培育对有害病毒有抗性的甜菜品种.
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