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在进化后,产生红醇的Yarrowia lipolytica对热应激的适应性反应

Kai Xia^1,2,3, Yuqing Chen¹, Fangmei Liu¹

¹School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China.

Applied microbiology and biotechnology

|March 15, 2024

概括

适应性实验室进化和突变发生有效地培育了耐热的Yarrowia lipolytica. 参与胺和分支链氨基酸代谢的关键基因被确定为热应激耐受性的关键基因.

科学领域:

工业生物技术工业生物技术
微生物遗传学微生物遗传学
压力生理学压力生理学

背景情况:

在Yarrowia lipolytica中,耐热性对于经济高效的红醇工业生产至关重要.
了解耐热性的遗传和分子基础对于菌株改进至关重要.

研究的目的:

通过适应性实验室进化 (ALE) 和突变发生,开发耐热的Yarrowia lipolytica菌株.
阐明Y. lipolytica.体内耐热性背后的分子机制.

主要方法:

适应性实验室进化 (ALE) 与60Co-γ和紫外线辐射集成,用于菌株繁殖.
比较基因组学以确定进化菌株中的突变.
在热应力下进行基因表达分析.
通过过度表达对关键基因的功能验证.

主要成果:

热耐药的Y. lipolytica菌株 (HT34,HT36,HT385) 已经成功生成.
在与信号传导,转录和翻译调节相关的基因中发现了突变.
热应激调高了参与DNA修复,胺/类固醇合成和BCAA/FFA降解的基因,同时调低了糖解和酸盐循环.
在耐热菌株中抑制了红醇的产生;外源BCAA和大豆油增强了生长.
过度表达特定的上调基因在Y. lipolytica CA20中产生了耐热性.

结论:

与突变发生结合的ALE是一种有效的策略,用于培育耐热的Y. lipolytica.
全球调节者的突变和代谢基因的差异表达是热适应的关键.
胺和分支链氨基酸 (BCAA) 对Y. lipolytica耐受热应激至关重要,为未来的菌株工程提供了目标.

关键词:

雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.适应性实验室进化的演变.分支氨基酸是分支的氨基酸.陶胺是一种陶胺.转录组转录组就是一个转录组.

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