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全基因组协会研究揭示了林木松的多基因架构定量疾病抵抗白松囊的耐药性

Jun-Jun Liu¹, Richard A Sniezko², Sydney Houston¹

¹Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.

|March 15, 2024

概括

在松中发展持久的抗病能力是生态系统恢复的关键. 这项研究使用全基因组关联来识别定量性疾病耐药性 (QDR) 的遗传标记,以对白松泡生.

科学领域:

森林遗传学森林遗传学
植物病理学植物病理学
生态系统恢复恢复生态系统

背景情况:

对各种病原体的持久耐药性对于恢复因白松泡生等疾病而受损的生态系统至关重要.
林柏松 (Pinus flexilis) 具有定量性抗病能力 (QDR),这是一个由多个基因影响的复杂特征.

研究的目的:

为了剖析松QDR到Cronartium ribicola的遗传结构.
识别与耐药性特征相关的特定遗传标记和基因位点.

主要方法:

全基因组关联研究 (GWAS) 针对用Cronartium ribicola.接种的18个月大的松苗.
在4年内对QDR特征 (针斑,干癌,存活率) 的定量评估.
针对性测序用于对抗疾病基因中的单核酸多态基因型 (SNPs) 的基因定型.

主要成果:

GWAS确定了与QDR特征相关的显著SNP,包括针点,干癌和生存率.
在12个链接组 (LGs) 中,在10个链接组中观察到标记特征关联的多基因模式.
在LG05上绘制了一个主要的抗性定量特征位点 (QTL),解释了瘤和瘤感染的表型变异高达52%.

结论:

该研究确定了松QDR的新型QTL,与之前绘制的主要基因耐药性不同.
与耐药性相关的候选基因包括NBS-LRR,LRR-RK和细胞染色体P450蛋白.
这些发现扩大了培育耐性松生殖基因质的遗传资源.

关键词:

候选基因是指一个候选基因.一个防御机制的防御机制.耐久性疾病耐药性耐药性具有耐受性的QTL.有针对性的安普利康序列测序.

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