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从使用非线性显微镜的档案脏活检中检索数据.

Lucas C Cahill1,2, Tadayuki Yoshitake2, Milan Rosen3

  • 1Harvard-MIT Division of Health Sciences and Technology, Harvard Medical School and Massachusetts Institute of Technology, Cambridge, MA, United States of America.

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概括

非线性显微镜 (NLM) 和光学清除允许从档案块中对脏活检进行3D成像. 这种技术增强了病理学检查,并使病的大规模回顾性研究成为可能.

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科学领域:

  • 腎臟病學 (nephrology) 是一種醫學專業.
  • 生物医学成像技术 生物医学成像技术
  • 病理学 病理学 病理学

背景情况:

  • 了解病依赖于彻底的活检检查.
  • 传统的方法涉及微粒体切割,这可能是限制性的.
  • 档案组织块为回顾性研究提供了宝贵的资源.

研究的目的:

  • 评估光非线性显微镜 (NLM) 结合光学清除用于活检的3D可视化的实用性.
  • 评估使用这种技术分析档案脏氨酸块的可行性.
  • 确定NLM是否可以提供对病理学的定量见解.

主要方法:

  • 档案中的脏石块被脱并染色.
  • 使用基醇基酸 (BABB) 进行了光学清除.
  • 非线性显微镜 (NLM) 用于对整个活检片段的3D成像,并将信号映射到H&E颜色尺度上.

主要成果:

  • 实现了病理的三维 (3D) 可视化,而无需微粒切割.
  • 诸如囊,球粒,排泄性病变和金梅尔斯蒂尔-威尔逊结节等结构在3D中进行了细分.
  • 获得了关于病理特征体积,直径和百分比组成的定量数据,观察到高质细胞数量.

结论:

  • 快速的光学清除和NLM成像使脏活检的更全面的检查成为可能.
  • 这种技术是分析档案石块的一个有前途的工具.
  • 档案块可以作为一个重要的资源,在科大规模的回顾性研究.