Kidins220和Aiolos通过减少TCR信号来促进胸膜iNKT细胞的发育
Laurenz A Herr1,2,3, Gina J Fiala1,2,3,4, Sagar5
1Signaling Research Centers BIOSS and CIBSS; University of Freiburg, Freiburg, Germany.
Science advances
|March 15, 2024
概括
220千达尔顿的Kinase D相互作用基质 (Kidins220) 在T细胞发育中发挥双重作用,在早期阶段降低T细胞受体 (TCR) 信号的调节,并在后期阶段促进它.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- T细胞发育是由T细胞受体 (TCR) 信号强度调节的.
- 支架蛋白Kidins220在T细胞发育中的作用以前是未知的.
研究的目的:
- 研究Kidins220在T细胞发育中的功能.
- 阐明Kidins220调节TCR信号的机制.
主要方法:
- 产生T细胞特异性的Kidins220淘汰赛 (T-KO) 小鼠.
- 在T-KO小鼠中分析T细胞群 (iNKT和常规T细胞).
- scRNA-seq用于识别下游效应器.
- 艾洛斯的淘汰赛分析.
主要成果:
- 在T-KO小鼠中,自然杀手T (iNKT) 细胞数量显著减少,常规T细胞数量略有减少.
- Kidins220降低了在发育中的iNKT细胞 (2和3阶段) 中的TCR信号的调节,在没有它的情况下导致增强的亡.
- 艾奥洛斯被确定为Kidins220在iNKT细胞发育中的下游效应剂.
- 来自T-KO小鼠的外围iNKT细胞显示TCR信号减少,表明Kidins220促进了外围的TCR信号.
结论:
- 在iNKT细胞发育过程中,Kidins220表现出TCR信号的阶段依赖调节.
- Kidins220作为一个关键的调节器,调节TCR信号强度,以确保适当的T细胞发育和功能.
更多相关视频
08:37Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
Published on: February 15, 2021
4.1K
09:41Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
Published on: July 26, 2024
754
相关概念视频
Cytotoxic T Cells-mediated Immune Response
907
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
907
T Cell Activation and Clonal Selection
726
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
726
Cells of the Innate Immune Response
1.6K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.6K
T Cell Types and Functions
1.0K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.0K
The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K
