Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hirsutine Ameliorates High-Fat Diet-Induced Cardiomyopathy Through Promoting LRPPRC-Regulated Mitophagy via the PINK1/Parkin Axis in Mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Eye tracking with a diffractive AR waveguide.

Optics letters·2026
Same author

Model-Driven Deep Learning Enables Speckle-Free Holography for 3D Parallel Nanofabrication.

Research (Washington, D.C.)·2026
Same author

Single-shot, reference-less computational wavefront sensing for complex optical fields.

Light, science & applications·2026
Same author

Phase-multiplied interferometry via cavity dynamics for resolution-enhanced coherent ranging.

Light, science & applications·2026
Same author

Spatial photometric information prediction based on back propagation artificial neural network.

Optics express·2025

相关实验视频

Updated: Jun 30, 2025

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

17.7K

基于阵列照明和子像素移位搜索的超高分辨率无透镜芯片上显微镜.

Yulong Li, Jianshe Ma, Liangcai Cao

    Optics letters
    |March 15, 2024
    PubMed
    概括

    本研究介绍了一种超高分辨率的无镜头芯片上显微镜系统,使用阵列光源和子像素转移搜索. 这种新的方法可以实现高分辨率的全息重建,而无需机械元件.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 显微镜的使用方法
    • 图像处理 图像处理

    背景情况:

    • 没有镜头的芯片内显微镜分辨率受到图像传感器像素大小的限制.
    • 现有的系统往往需要机械元件和精确的校准.

    研究的目的:

    • 引入超高分辨率的芯片上显微镜系统,克服像素大小的限制.
    • 开发用于快速超分辨率全息重建的算法.
    • 为了消除对位移控制平台和照明角度校准的需求.

    主要方法:

    • 使用一个紧的阵列光源与四个连续照亮RGB LED模块.
    • 实现基于全息图频率比较的子像素移位搜索算法.
    • 使用超分辨率重建算法与多波长相位检索.

    主要成果:

    • 在部分连贯照明下实现超分辨率相位重建.
    • 演示了一个系统,避免了对位移控制平台的需求.
    • 能够在一个感兴趣的区域内快速超分辨率重建全息图.

    结论:

    • 拟议的系统和算法有效地提高了无透镜芯片显微镜的分辨率.

    更多相关视频

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.5K
    Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
    08:19

    Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

    Published on: December 14, 2009

    12.0K

    相关实验视频

    Last Updated: Jun 30, 2025

    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    17.7K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.5K
    Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
    08:19

    Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

    Published on: December 14, 2009

    12.0K
  • 该方法可方便在没有复杂设置的情况下进行实用的超分辨率全息成像.
  • 这一进步为紧的,高分辨率的显微镜成像应用提供了可能性.