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相关概念视频

Lysogenic Cycle of Bacteriophages00:43

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Antibiotic Selection

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Overview
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相关实验视频

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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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发酵实践 在菌体中选择热稳定的内素.

Frank Oechslin1,2,3, Xiaojun Zhu1,2, Carlee Morency1,2,3

  • 1Département de biochimie, de microbiologie, et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec City, Canada.

Molecular biology and evolution
|March 15, 2024
PubMed
概括

菌体内素向Streptococcus thermophilus显示有限的多样性. 一种新的结合基因增强了酶的稳定性和活性,表明乳制品发酵环境中的积极选择.

关键词:
这种菌株是Streptococcus thermophilus.细菌菌体是一种细菌体.奶制品发酵 乳制品发酵这种内分泌物是endolysins.实验进化的实验进化.宿主适应 宿主适应

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科学领域:

  • 微生物学 微生物学
  • 酶学 是一种酶学.
  • 进化生物学 进化生物学

背景情况:

  • 菌体内素对于细菌细胞壁溶解和菌体释放至关重要.
  • 内分泌体表现出多样化的模块化结构,但进化驱动因素仍然不清楚.
  • 乳制品发酵剂Streptococcus thermophilus提供了一个专注的生态利基,以研究内的进化.

研究的目的:

  • 研究从感染Streptococcus thermophilus的菌体中产生的内素的多样性和进化.
  • 确定S. thermophilus endolysins的结构特征及其功能意义.
  • 确定是否受乳制品发酵的特定环境条件的影响.

主要方法:

  • 来自S. thermophilus菌体的内素的生物信息分析.
  • 已识别的内素的结构和功能特征.
  • 在不同温度和度下进行酶活性测定.
  • 对内素基因的积极选择压力的分析.

主要成果:

  • 在S. thermophilus菌体中观察到有限的内素多样性,具有主导的结构类型.
  • 在普遍存在的内素结构中发现了一种新的,保存的结合基因.
  • 这种模式显著提高了在高温下酶的稳定性和活性.
  • 在模仿乳制品发酵的条件下,证明了这种模式的积极选择.

结论:

  • 在S. thermophilus中,内素的进化受到限制,有利于适应乳制品环境的特定结构类型.
  • 鉴定出的结合基因是高温发酵中内素功能的关键适应.
  • 这一发现突显了生态利基和环境因素在塑造菌体编码酶进化的作用.