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探索基于单细胞高通量药物查平台的化学敏感性接触依赖的细胞-细胞通信机制

Yue Jiang¹, Xuelian Ren², Guobin Liu²

¹School of Mechanical Engineering and Automation, Northeastern University, Shenyang, 110819, China; State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

|March 15, 2024

概括

我们开发了一种用于单细胞高通量药物查 (HTDS) 的微流体芯片,以研究细胞通信如何影响癌症药物疗效. 这个平台揭示了阻断细胞通信可以增加化学抵抗,但也可以抑制瘤生长.

科学领域:

生物医学工程生物医学工程
癌症研究癌症研究
药理学药理学是指药理学的学科.

背景情况:

高通量药物查 (HTDS) 加快了药物开发,但可能会忽视细胞间通信效应.
接触依赖的细胞与细胞通信 (CDCCC) 可以影响瘤细胞的化学敏感性.
需要具有成本效益的单细胞HTDS平台,并了解CDCCC对药物疗效的影响.

研究的目的:

为单细胞HTDS开发一个微流体芯片.
研究CDCCC影响瘤细胞化学敏感性的分子机制.
评估CDCCC在药物疗效和瘤进展中的作用.

主要方法:

开发一种微流体芯片,用于精确的药物混合和单细胞捕获.
对芯片性能与96井板测试的验证.
基于定量质谱的蛋白质组学来分析分子变化.
路径丰富分析以确定受影响的信号路径.

主要成果:

微流体芯片展示了高质量的单细胞捕获和药物查,与传统方法相一致.
在单个瘤细胞中缺少CDCCC可以增强化疗抵抗性,但也会导致更大的增殖抑制.
蛋白质组分析显示,CDCCC对调节瘤增殖,粘附和入侵的信号通路产生影响.

结论:

CDCCC显著影响瘤细胞的化学敏感性和生物过程.
开发的单细胞HTDS平台为CDCCC介导的耐药性提供了洞察力.
这项研究支持个性化癌症治疗策略和单细胞查技术的发展.

关键词:

取决于接触的细胞与细胞通信.高通量药物查的高通量药物查微流体芯片是一个微流体.量化蛋白质组学是一种定量化的蛋白质组学.瘤单细胞的一个细胞.

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