染色体激活与H3K36me2和对转移性割抵抗性前列腺癌的隔间移动
Sanji Kanaoka1, Atsushi Okabe2, Manato Kanesaka1
1Department of Molecular Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan; Department of Urology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Cancer letters
|March 15, 2024
概括
增加NSD2的表达驱动致命的前列腺癌,通过改变质子修饰和染色质结构,促进瘤的生长和复发. 这种表观遗传重编程对于抵抗割的前列腺癌进展至关重要.
科学领域:
- * 瘤学 瘤学是一门专业.
- * 表观遗传学 是一种表观遗传学.
- * 分子生物学 * 分子生物学
背景情况:
- *表观遗传修饰剂在抵抗割的前列腺癌 (CRPC) 中被上调,导致治疗耐药性.
- * 在CRPC进展中,基因组修饰和染色质结构之间的相互作用仍然不完全理解.
研究的目的:
- * 调查表观遗传重编程在CRPC中的作用.
- * 在CRPC的发展和进展中确定关键的表观遗传修饰剂及其下游目标.
主要方法:
- *重新分析临床转录组和结果数据.
- * 染色体免疫沉测序 (ChIP-seq),RNA测序 (RNA-seq) 和Hi-C用于表观基因组和转录基因组分析.
- * 识别不同调节的基因和染色体组件.
主要成果:
- * NSD2,一种组分蛋白甲基转移酶,在CRPC中被确定是上调调的,并且与更高的复发率相关.
- *NSD2的上调导致H3K36me2的增加和H3K27me3的减少,将染色质从非活性转移到活性区.
- *68个异常激活的基因被确定为NSD2的下游目标,KIF18A被认为对CRPC生长至关重要.
结论:
- * NSD2介导的表观遗传变化,包括H3K36me2的增加和H3K27me3的损失,是CRPC的关键驱动因素.
- * 合作性基因组修饰和染色质结构的变化驱动前列腺癌发生.
- *KIF18A是CRPC的一个潜在的治疗点.
更多相关视频
相关概念视频
Heterochromatin
12.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.6K
Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
Euchromatin
6.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.9K
Chromatin Position Affects Gene Expression
23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K


