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斯波里索里亚米兰 (Sporisorium reilianum) 聚糖通过调节肠道屏障功能和代谢物来改善DSS诱导的性结肠炎

Shuaichen Liu¹, Lan Hong², Song Zhang³

¹Department of Food Science and Engineering, College of Agricultural, Yanbian University, Yanji 133000, Jilin, China; Department of Food Science and Engineering, College of Integration Science, Yanbian University, Yanji 133000, Jilin, China.

International journal of biological macromolecules

|March 15, 2024

概括

聚糖体Sporisorium reilianum (SRPS) 显示出保护肠道屏障的潜力. 这些化合物改善了肠道健康,并调节了大肠炎小鼠的新陈代谢,减少了炎症和损伤.

科学领域:

胃肠病学胃肠病学
免疫学免疫学免疫学
代谢学代谢学代谢学

背景情况:

性结肠炎 (UC) 是一种慢性炎症性肠病,其特征是肠壁功能障碍和代谢失调.
硫酸德克斯 (DSS) 诱导的小鼠结肠炎是一种广泛使用的模型,用于研究UC病变和治疗干预措施.
聚糖体 (Sporisorium reilianum polysaccharides,简称SRPS) 是具有潜在免疫调节和代谢作用的生物活性化合物.

研究的目的:

为了研究SRPS对肠道屏障功能和代谢的调节作用,在DSS诱导的大肠炎的小鼠模型中.
评估SRPS对疾病活动,结肠组织学以及炎症和屏障完整性的关键分子标记物的影响.
探索SRPS对肠道微生物群和相关代谢途径的影响.

主要方法:

向患有DSS诱导的大肠炎的小鼠给予SRPS.
评估疾病活动指数 (DAI) 和结肠长度.
结肠组织的组织病理学检查,包括杯细胞计数和粘素分泌.
对促炎性细胞因子表达的分析 (例如TNF-α,IL-6).
代谢学分析,以确定代谢物水平的变化.
对紧密结合蛋白 (例如,奥克卢丁,ZO-1) 和与亡相关的蛋白质 (Bcl-2,Bax) 的评估.
测量短链脂肪酸 (SCFA) 和SCFA受体的激活 (例如GPR41).

主要成果:

在DSS治疗的小鼠中,SRPS显著降低了疾病活性指数和结肠缩短.
施用SRPS使结肠中促炎性细胞因子的表达正常化.
治疗SRPS导致有益的短链脂肪酸 (乙酸,酸,黄油酸) 的水平增加.
代谢分析显示,由于DSS,氨基酸生物合成,酸代谢和其他途径发生了显著的变化,这些变化是由SRPS调节的.
SRPS减少了杯状细胞的损失,增强了粘素分泌,调高了紧结蛋白 (occludin,ZO-1),并抑制了上皮细胞的亡.
观察到SCFA受体GPR41的激活和NLRP3炎症组分水平的降低.

结论:

聚糖体Sporisorium reilianum在改善小鼠DSS诱导的大肠炎方面显示出显著的治疗潜力.
通过加强肠道屏障,调节宿主新陈代谢和减少结肠炎症,SRPS具有保护作用.
这些发现表明,SRPS可能是治疗性结肠炎的有希望的候选人.

关键词:

肠道屏障肠道屏障肠道屏障代谢学代谢学代谢学聚糖类是一种多糖类.

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