由Beauveria bassiana使用基于生物膜的两步发酵方式高效地生产R-2-(4-基基) 酸
Shuping Zou1, Yizhi Ma1, Wei Ding1
1National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014, PR China; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Bioresource technology
|March 15, 2024
概括
使用Beauveria bassiana的新型生物膜发酵有效地将R-2-基酸 (R-PPA) 转化为R-2-基酸 (R-HPPA),显著提高了生产产量.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物催化剂是一种生物催化剂.
背景情况:
- 传统的潜水发酵限制了R-2-(4-基氧) 酸 (R-HPPA) 的产生.
- 贝沃里亚巴西安纳是一种具有生物转化应用潜力的真菌.
- 优化发酵条件对于提高微生物产品产量至关重要.
研究的目的:
- 开发和优化一种基于生物膜的新发酵工艺,用于生产R-HPPA.
- 为了比较生物膜发酵的效率与传统的浸泡发酵.
- 研究关键因素对R-HPPA合成和生物膜形成的影响.
主要方法:
- 使用Beauveria bassiana的生物膜培养模型进行R-PPA转换.
- 优化了曼尼托尔剂量,试剂量和生物膜形成和R-HPPA合成的初始pH值.
- 开发了一种两级生物反应器,将振动和静态化相结合,以提高生产效率.
主要成果:
- 与浸泡发酵相比,生物膜培养显著增加了R-HPPA标位.
- 在最佳条件下,R-HPPA的产量为22.2g/L,生产率为3.2g/L·d.
- 两级生物反应器实现了100%的R-PPA转化,达到50g/L的R-HPPA,生产率为3.8g/L·d.
结论:
- 基于生物膜的发酵是R-HPPA生产的优越策略.
- 开发的两阶段生物反应器工艺显示出对工业规模R-HPPA合成的前景.
- 这种方法提供了一种生产基化芳香化合物的可行方法.
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