优化玻璃珠灭菌协议,重点消除有机和细菌手术内污染
Keith Lewy1, Jonathan Bova1, Timothy A Erickson2
1Comparative Medicine Program, Texas A&M University, College Station, Texas.
概括
机械清洁对于在细菌污染后消毒动物手术仪器至关重要. 使用洗剂或化的预处理,加上刷牙,显著改善不育性,减少有机物质.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 手术仪器灭菌手术仪器灭菌
背景情况:
- 需要经过验证的协议,用于在细菌暴露后杀菌动物手术仪器.
- 目前的方法可能无法有效地去除生物残留物,从而损害无菌性.
研究的目的:
- 为了评估不同预处理方法的有效性,结合玻璃珠灭菌用于动物手术仪器.
- 为了确定最佳的协议,以实现仪器无菌性,污染后的内含量.
主要方法:
- 动物手术仪器 (子,针驱动器) 被污染了的内容和空气干燥.
- 仪器经过预处理 (多酶洗剂,中性pH洗剂,赫西丁擦拭剂或盐水),然后进行刷毛.
- 使用玻璃珠灭菌器 (60秒在500°F/260°C) 实现了灭菌.
- 使用细菌培养和ATP生物发光测试来评估无菌性和有机物质的存在.
主要成果:
- 机械刷毛对于清除碎片和仪器无菌性至关重要.
- 当与刷牙相结合时,预处理剂进一步减少了与单独盐水相比的有机污染.
- 在所有组中都观察到最小的细菌生长,只有盐水组显示轻微的污染.
结论:
- 通过刷子进行机械清洁对于有效消毒受污染的动物手术仪器至关重要.
- 加入洗剂或赫西丁等预处理溶液可以增强有机物质的去除,并改善整体无菌性.
- 机械清洁和适当的化学预处理的结合提供了一个强大的灭菌协议.
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