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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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相关实验视频

Updated: Jun 30, 2025

Robust 3D DNA FISH Using Directly Labeled Probes
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使用多个共同表达的基因的FISHnCHIPs的高度敏感的空间转录组学.

Xinrui Zhou1, Wan Yi Seow1, Norbert Ha1

  • 1Genome Institute of Singapore, Agency for Science, Technology and Research (A*STAR), 60 Biopolis Street, Singapore, 138672, Singapore.

Nature communications
|March 16, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了光现场混合细胞异质性和基因表达程序 (FISHnCHIPs),这是一个敏感和高效的方法,用于空间奥米克. 为了研究和诊断,FISHnCHIPs能够在组织中快速,高分辨率的细胞类型.

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相关实验视频

Last Updated: Jun 30, 2025

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科学领域:

  • 生物医学研究的研究.
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 现有的空间奥米克技术昂贵且耗时.
  • 高维,空间分辨的组织分析对于研究和诊断至关重要.

研究的目的:

  • 引入光现场混合细胞异质性和基因表达程序 (FISHnCHIPs),用于敏感的现场细胞类型和基因表达概况.
  • 为了克服当前空间奥米克技术的局限性.

主要方法:

  • FISHnCHIP同时对~2-35个共同表达的基因进行图像,这些基因被聚集成模块.
  • 它实现了高灵敏度和空间分辨率,与单基因FISH相美.
  • 该方法允许高速,大视野组织分析.

主要成果:

  • FISHnCHIPs表现出比传统的FISH高出2-20倍的灵敏度.
  • 在小鼠脏和大脑组织中成像了多达53个模块.
  • 在结直肠癌活检中确定了与癌症相关的空间受限纤维细胞.

结论:

  • FISHnCHIPs提供了一种快速,强大的,可扩展的解决方案,用于组织中的细胞类型.
  • 这项技术推进了在正常生理学和疾病发病过程中对组织的研究.
  • FISHnCHIPs对生物医学研究和诊断有重大影响.