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在小麦 (Triticum aestivum L.) 中确定和验证主要和稳定的定量特征位点,以确定小麦 (Triticum aestivum L.) 的数量

Zhi Li^1,2,3, Qinyi Luo^2,3, Yujie Gan²

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|March 16, 2024

概括

一个主要和稳定的定量特征位点 (QTL),QFn.sau-1B.2,被确定为小麦降落数 (FN),一个关键的质量特征. 这种QTL显著影响FN,并有助于抵抗尖发芽,为小麦育种提供了宝贵的资源.

科学领域:

植物遗传学和育种.
农业科学农业科学
分子生物学分子生物学

背景情况:

下降数 (FN) 是一个重要的小麦谷物质量特征,也是全球小麦贸易的主要指数.
识别影响FN的遗传因素对于提高小麦质量和贸易价值至关重要.

研究的目的:

绘制和验证与小麦下降数 (FN) 相关的定量特征位置 (QTL).
确定FN的稳定和主要QTL,并调查它们的遗传影响.

主要方法:

在三年内使用了包括性复合间隔映射 (ICIM) 方法 (ICIM-BIP,ICIM-MET,ICIM-EPI).
使用竞争性等位基特异性PCR (KASP) 标志物KASP-AX-108743901用于标志物辅助选择.
在不同小麦种群中验证了已识别的QTLs的遗传影响.

主要成果:

在染色体1BL上发现了一个主要且稳定的QTL,QFn.sau-1B.2,解释了FN.的13.6%的表型变异.
QFn.sau-1B.2 显示了对 FN 的显著添加和表观效应,并积极影响了对尖发芽的抵抗力.
根据注释和转录组数据,在QFn.sau-1B.2区间内的四个候选基因可能与FN相关.

结论:

QFn.sau-1B.2是一种有价值的遗传资源,可以提高小麦的下降数量和质量.
已识别的QTL为旨在改善小麦质量特征的标记器辅助育种计划提供了潜力.
对候选基因的进一步研究可以阐明在小麦中FN确定背后的分子机制.

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