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基于网络药理学,生物信息学分析和实验验证的基础上,在类风湿性关节炎中识别了Hedyotis diffusa Willd特定的mRNA-miRNA-lncRNA网络

Jing Jiang¹, Meng Huang¹, Si-Si Zhang¹

¹School of Laboratory Medicine, Chengdu Medical College, Chengdu, 610500, Sichuan, China.

Scientific reports

|March 16, 2024

概括

威尔德 (Hedyotis diffusa Willd) 可能通过调节MMP9/miR-204-5p/MIAT轴来治疗类风湿性关节炎 (RA). 这项研究确定了MMP9作为关键基因,并证实了HDW.

科学领域:

综合性瘤学和系统药理学.
传统中国医学 (TCM) 的研究.
分子生物学和生物信息学.

背景情况:

类风湿性关节炎 (RA) 是一种具有复杂病原性的慢性炎症性疾病.
(Hedyotis diffusa Willd,HDW) 是一种传统的中医药,具有已知的抗炎和抗癌特性.
在RA中HW的治疗机制尚不清楚.

研究的目的:

为了研究HDW在类风湿性关节炎中的潜在治疗点和分子机制.
构建HDW组件,目标和RA相关基因的监管网络.
为了确定关键的基因和途径,涉及到HDW对RA的作用.

主要方法:

对基因表达数据集的生物信息分析 (GEO GSE77298).
构建药物-成分-向疾病和蛋白质-蛋白质相互作用 (PPI) 网络.
机器学习算法 (LASSO,RF,SVM-RFE) 用于核心基因识别.
对mRNA-miRNA-lncRNA调节网络 (ceRNA) 的预测.
在RA-FLS细胞和分子对接中进行实验验证.

主要成果:

MMP9被确定为与RA诊断和免疫细胞透相关的核心基因.
高血压证明了RA-FLS细胞增殖和MMP9表达的剂量依赖抑制.
预测MMP9/miR-204-5p/MIAT轴是RA中HW的关键调节途径.
分子对接证实了HDW活性成分和MMP9.9之间的相互作用.

结论:

通过调节MMP9/miR-204-5p/MIAT ceRNA网络,HW可以在RA中产生治疗效果.
MMP9 是一种潜在的治疗点,用于治疗HW的RA.
这项研究为了解RA治疗中的TCM机制提供了一个新的框架.

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