吉伯雷林通过二元菜中的SpGAI-SpSTM模块来调节男性化
Yu-Lan Zhang1, Li-Ying Wang1, Yi Yang1
1College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.
The Plant journal : for cell and molecular biology
|March 16, 2024
概括
吉伯酸 (GA) 应用将雌性菜植物转化为雄性,揭示了一条新的途径. 这涉及GA信号抑制剂SpGAI与SpSTM相互作用以抑制SpPI,控制双植物的性别决定.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生殖生物学 生殖生物学
背景情况:
- 双胞胎植物的性别主要是遗传的,但可以被植物激素等环境因素改变.
- 已知Gibberellins (GA) 影响了花期和性发育,但它们在菜中性别转换中的作用尚不清楚.
研究的目的:
- 为了阐明在双胞胎菜 (Spinacia oleracea L.) 中由吉伯雷林诱导的性别转换的分子途径.
- 调查SpGAI和SpSTM在GA介导的性别决定中的作用.
主要方法:
- 将GA3应用于雌性菜植物,并使用分子标记 (T11A,SSR19) 评估性别转换.
- 对SpGAI和SpSTM进行基因沉默,以观察对性别决定的影响.
- 分析SpGAI和SpSTM之间的物理相互作用.
- 调查SpSTM与SpPI内子的结合及其对SpPI表达的影响.
主要成果:
- 外源性GA3应用成功诱导了雌性菜植物的完全男性化,从而产生了功能性树干.
- 沉默SpGAI,一个DELLA抑制剂,模仿了GA的男性化效应.
- SpGAI与KNOX转录因子SpSTM进行物理相互作用.
- 沉默SpSTM也导致了雌性花的男性化.
- 通过与其内核结合,SpSTM直接抑制SpPI的表达.
结论:
- 通过SpGAI-SpSTM-SpPI调节模块,GA诱导了菜中的女性到男性的性别转换.
- 这一途径澄清了双胞胎植物中性别决定和单性花的发展的分子机制.
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