相关实验视频

Updated: Apr 12, 2026

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Published on: May 12, 2017

由于低温引起的核应激下,大鼠肝细胞核中的结构变化

I P Bobrov¹, A Yu Dolgatov¹, A V Lepilov¹

¹Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia.

Bulletin of experimental biology and medicine

|March 16, 2024

概括

深度低温会诱导大鼠肝细胞中的核应激,其特征是纤维细胞中心和核膜结构的减少. 过渡性核细胞增加,表明在暴露在寒冷中存在补偿储备机制.

科学领域:

细胞生物学细胞生物学
生理学生理学生理学
低温研究研究低温研究

背景情况:

核对核糖体生物发生和细胞应激反应至关重要.
低温显著影响细胞结构和功能.
了解低温期间的核动力学是细胞生存的关键.

研究的目的:

在深度低温下研究大鼠肝细胞核中的结构变化.
识别核细胞组件的特定变化,如纤维细胞中心和核细胞膜结构.
探索核细胞对寒冷压力的适应性反应.

主要方法:

在老鼠中,深度低温引起5°C水浸浸40分钟.
分析肝细胞核的结构和组成.
核细胞组成部分 (纤维细胞中心,核细胞膜和过渡核细胞) 的定量评估.

主要成果:

纤维细胞中心 (FC) 和每个核和每个核细胞的核细胞核显著减少.
过渡型核细胞的增加,每个核的自由FC,以及自由FC的百分比.
观察到核子应激现象与补偿性适应反应.

结论:

深度低温会在老鼠肝细胞中引发核应激.
过渡核细胞和增加的自由FC表明存在一个作为适应机制的储备核细胞池.
这些发现提供了关于低温期间细胞弹性的见解.

关键词:

肝细胞肝细胞肝细胞低温症低温症是一个原子核组织者原子核组织者核子压力是核子中的压力.笔记 - 打印 - 印记

更多相关视频

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Published on: November 14, 2018

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

相关概念视频

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...