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Carmen Dost¹, Florian Michling¹, Davies Kaimenyi²

¹Department of Microbiology and Biochemistry, Hochschule Geisenheim University, Von-Lade-Strasse 1, Geisenheim 65366, Germany; Geisenheim Yeast Breeding Center, Hochschule Geisenheim University, Von-Lade-Strasse 1, Geisenheim 65366, Germany.

Microbiological research

|March 16, 2024

概括

从环境样本中分离出Saccharomycopsis酵母,由于缺乏硫酸盐载体,耐毒酸盐. 这些酵母表现出潜在的掠食性生物体,其中包括S. schoenii和一种新物种S. geisenheimensis sp. nov., 展现同质生殖的生殖.

科学领域:

微生物学微生物学
酵母遗传学酵母遗传学
生物技术是生物技术.

背景情况:

萨卡罗米科普西斯物种是天然的有机硫辅食物,缺乏硫酸盐吸收和同化基因.
由于共享的运输通路,这种缺陷使得对酸盐产生耐药性,酸盐是许多微生物的毒性化合物.
这种独特的特征被利用用于选择性丰富和分离Saccharomycopsis物种.

研究的目的:

从环境样本中分离和描述新的Saccharomycopsis物种,使用酸盐耐药性作为选择性标记.
评估孤立的Saccharomycopsis物种的掠食潜力和繁殖策略.
为了确认不同Saccharomycopsis分离物的干扰性和分类学地位.

主要方法:

选择性丰富环境样本使用酸盐作为有毒剂.
酵母物种的隔离和识别,重点是Saccharomycopsis.
在不同的温度下进行生长试验和使用S. schoenii和S. cerevisiae作为猎物进行掠食试验.
用标记器指导的繁殖交叉来评估干扰性和繁殖相容性.

主要成果:

分离了S. schoenii,S. lassenensis,以及一个新物种,S. geisenheimensis sp. 新的,与其他酵母的最小污染.
S. lassenensis菌株耐受37°C,与大多数Saccharomycopsis物种不同.
捕食活动在20°C时较高,而S. schoenii. 在30°C时较高.
成功的生育交叉证实了S. schoenii分离物属于同一生物物种.
证实S. lassenensis是异质的,而S. schoenii和S. geisenheimensis sp.则是不同的. Nov. 是同质的.

结论:

耐酸盐是一种有效的方法,可以从复杂的环境样本中分离Saccharomycopsis物种.
这种新分离的S. geisenheimensis sp. 新的和特征的S. schoenii和S. lassenensis表现出不同的生理和生殖特征.
萨卡罗米科普西斯物种,特别是S. schoenii,显示出潜在的掠食性酵母,其活动取决于温度.
了解繁殖策略 (同质与异质) 对这些酵母菌种的分类学和未来研究至关重要.

关键词:

繁殖繁殖繁殖繁殖异形的异形.同型的同型的 Homothallic 配对类型配对类型抗酸盐的耐药性抗酸盐的耐药性

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