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在早期流产和胎盘之间,与自发流产有关的HLA-G异型,HLA-C全型及其表达不同

Mayuri Bora¹, Sushmita Singha², Taruna Madan³

¹Department of Molecular Biology and Biotechnology Tezpur University, Napaam, Sonitpur, Assam, 784028, India.

|March 16, 2024

概括

自发流产可能与特定的免疫相互作用有关. 自发流产样本中KIR2DS1+/2DL1+ HLA-C2的较低水平和HLA-G3异型的较高比例表明它们在流产中的作用.

科学领域:

生殖免疫学生殖免疫学
母胎界面母胎界面
怀孕并发症怀孕并发症

背景情况:

自发流产 (SAB) 影响约10%的怀孕,可能与母胎界面的免疫功能障碍有关.
胎儿热囊细胞入侵和螺旋动脉重塑涉及热囊细胞HLA-G/HLA-C和状NK细胞KIR之间的交叉声.

研究的目的:

为了研究HLA-G异型,KIR2D和HLA-C在自发流产中的关联.
为了探索第一个三个月的流产和长期胎盘之间的这些免疫因素的差异.

主要方法:

分析HLA-G,KIR2D和HLA-C在母亲-新生儿对,选择性流产,SAB流产和长期胎盘中.
使用SSP-PCR进行基因定型,RT-PCR用于基因表达,ELISA用于蛋白质表达.
包括对HLA-C.的副本数变异 (CNV) 分析.

主要成果:

与选择性流产 (可溶性异型) 相比,SAB流产显示HLA-G3异型的比例更高 (50%).
长期的胎盘中,膜结合的HLA-G4的比例更高 (39.28%).
SAB与较高的抑制KIR2DL1和较低的HLA-C2有关,与健康的胎盘相比,KIR2DS1和KIR2DS1+/2DL1+-HLA-C2基因型较高,KIR2D和HLA-C2蛋白水平较高.

结论:

较低的KIR2DS1+/2DL1+-HLA-C2的组合和HLA-G3异型的优势可能会导致自发流产.
早期堕胎和胎盘之间的HLA-G异型和表达的差异表明时间调制,并突出了这项研究的新方面.

关键词:

在CNVs中,可以看到CNVs.选择性流产是一种选择性流产.在HLA-G的同位体中.基因组合基因型KIR,HLA-C组合基因型在SAB中,堕胎就是堕胎.自发的怀孕流产怀孕流产

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