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Updated: Jun 30, 2025

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

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微生物进化工程的突变基因技术 - 利用CRISPR-Cas,寡核酸,重组酶和聚合酶

Anna Zimmermann1, Julian E Prieto-Vivas1, Karin Voordeckers1

  • 1VIB Laboratory for Systems Biology, VIB-KU Leuven Center for Microbiology, Leuven, 3001, Belgium; CMPG Laboratory of Genetics and Genomics, Department M2S, KU Leuven, Leuven, 3001, Belgium.

Trends in microbiology
|March 16, 2024
PubMed
概括

进化工程通过促进有益的突变来增强工业应用中的微生物. 新的方法加速了高级微生物细胞工厂的发展,用于精密发酵.

关键词:
指导进化是指导进化的.工程微生物 工程微生物代谢优化 - 代谢优化精密发酵精密发酵的方法有针对性的突变发生.

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In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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