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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Jun 30, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

151

克里斯普尔激活屏幕:导航技术和应用程序

Teleri Clark1, Matthew A Waller1, Lipin Loo1

  • 1Charles Perkins Centre, Dr. John and Anne Chong Lab for Functional Genomics, and School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.

Trends in biotechnology
|March 16, 2024
PubMed
概括

克里斯普尔激活 (CRISPRa) 能够通过高通量基因选来识别在上调时引起特定表型的基因. 本综述涵盖了各种研究领域的CRISPRa技术和应用.

关键词:
在CRISPR屏幕上使用CRISPR.克里斯普拉是一个.激活基因激活 基因激活基因查 基因查 基因查

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物技术是生物技术.

背景情况:

  • 克里斯普尔激活 (CRISPRa) 是分子生物学中的一个关键工具.
  • 克里斯普拉使得高通量基因选能够识别负责特定表型的基因.
  • 正在进行的开发旨在提高CRISPRa的效率和一致性.

研究的目的:

  • 为CRISPRa架构提供技术概述.
  • 为了总结聚合的CRISPRa查方法.
  • 讨论CRISPRa查在研究中的当前和新兴应用.

主要方法:

  • 对CRISPRa技术的审查.
  • 汇集的CRISPRa查策略的总结.
  • 对当代研究应用的分析.

主要成果:

  • 现有CRISPRa系统的概述.
  • 聚合的CRISPRa屏幕的综合摘要.
  • 讨论各种研究应用.

结论:

  • 克里斯普拉查是一种强大的基因发现方法.
  • 该技术在科学学科中具有广泛的适用性.
  • 新兴的应用有望进一步推动生物研究的进步.